H8032
Hanahan′s Broth (SOB Medium)
powder microbial growth medium
Synonym(s):
Super Optimal Broth
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About This Item
UNSPSC Code:
41106200
NACRES:
NA.85
Recommended Products
grade
for molecular biology
Quality Level
description
recommended for cultivation of recombinant E. coli.
sterility
non-sterile
form
powder
composition
KCl, 0.186 g/L
MgSO4, 2.4 g/L
NaCl, 0.5 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2.8% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
General description
Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication.
Application
Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
Features and Benefits
Hannahan′s Broth (SOB) powder provides:
- Standard formulation
- An inexpensive alternative to liquid
Reconstitution
Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Other Notes
Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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