G006130
HPLC Analysis of E. coli Lysate on Proteomix® SAX-NP3, Demonstration of High Loading Capacity
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Proteomix SAX-NP3, 5 cm x 4.6mm I.D., 3 μm particles (Z777219)
mobile phase: (A) 20 mM Tris, pH 9.0; (B) A + 0.5 M NaCl
gradient: 0 - 100% B in 30 min
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: as indicated in figure
sample: 2.5 mg/mL
suitability
application for HPLC
application(s)
life science and biopharma
pharmaceutical (small molecule)
Legal Information
analytical column
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