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T8768

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

horseradish peroxidase substrate, chromogenic, ≥98%, powder

Synonym(s):

TMB dihydrochloride

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About This Item

Linear Formula:
[-C6H2(CH3)2-4-NH2]2·2HCl·xH2O
CAS Number:
207738-08-7
Molecular Weight:
313.27 (anhydrous basis)
MDL number:
MFCD00150104
UNSPSC Code:
12352204
PubChem Substance ID:
24900558
NACRES:
NA.83

product name

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate, powder, ≥98%

Assay

≥98%

form

powder

solubility

acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy

storage temp.

2-8°C

SMILES string

O.Cl.Cl.Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2

InChI key

KVCWTKDFVVSVSJ-UHFFFAOYSA-N

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General description

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.

Application

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).
Noncarcinogenic substitute for benzidine.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H302,H351,H413

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Detection of Antibodies Against DNA Polymerase of Hepatitis B Virus in HBsAg-Positive Sera Using ELISA
Rui LX, et al.
The Korean Journal of Internal Medicine, 13, 95-95 (1998)
Robot Printing of Reverse Dot Blot Arrays for Human Mutation Detection
Lappin S, et al.
The Journal of Molecular Diagnostics : JMD, 3, 178-178 (2001)
Quantitative determination of hemoglobin and cytochemical staining for peroxidase using 3, 3?, 5, 5?-tetramethylbenzidine dihydrochloride, a safe substitute for benzidine
Liem HH, et al.
Analytical biochemistry, 98(2), 388-393 (1979)
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