SRP7603

DNA Binding Protein-7 (DBP-7), recombinant

recombinant, expressed in E. coli, ≥99% (SDS-PAGE)

• Synonym(s): DNA Binding Protein 7
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Assay: ≥99% (SDS-PAGE)
• form: lyophilized
• mol wt: ~9.4 kDa
• packaging: pkg of 20 μg
• impurities: endotoxin, tested
• NCBI accession no.: P39476
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... sso7d(1454006)

Description

General description

DNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete β-barrel capped at the opening by a C-terminal α-helix; they bind to the minor groove of a DNA duplex via the triple-stranded β-sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein–protein interactions. In vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.

Physical form

Sterile filtered and Lyophilized without additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable