SCJ4600013

CF^™ 405S succinimidyl ester

suitable for fluorescence, ≥90% (TLC)

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• Quality Level: 100
• Assay: ≥90% (TLC)
• form: solid
• mol wt: ~1169 g/mol (free acid)
• solubility: DMF: soluble; DMSO: soluble; H₂O: soluble
• ε (extinction coefficient): 33000 at 404 nm
• absorbance ratio: A_{280/404 nm} 0.7
• fluorescence: λ_ex 360, 405 or 407 nm (Flow cytometry laser line); λ_ex 404 nm; λ_em 431 nm; λ_ex 405 nm (Microscopy laser line)
• suitability: suitable for fluorescence
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

CF^™ 405S, succinimidyl ester (CF405S SE), a blue, fluorescent dye, is used for labeling proteins or other biomolecules having an amine group. The succinimidyl ester group of the dye reacts with an amine group to form a stable amide linkage. It has an absorption peak wavelength that nearly coincides with the 405 nm blue diode laser line. In addition, the emission peak wavelength of the dye well centers within the blue detection window of BD flow cytometers. As a result, flow cytometry analysis using CF 405S dye results in much brighter signal than that using Alexa Fluor^™ 405.

Application

CF^™ 405S succinimidyl ester has been used:

• to label diphtheria toxoid (DT) to determine the percentage of DT associated with liposomes
• in the preparation of the dye-encapsulated silica nanoparticles (CF 405S SiNP)
• as a fluorescent dye for the fluorescent labeling of native human serum albumin (HSA) (HSAUT)

Features and Benefits

1. Much brighter than Alexa Fluor^™ 405 due to better compatibility with both excitation and emission windows on commom instruments. 2. Highly water-soluble. 3. pH-insensitive.

Preparation Note

Protect from light and moisture

Reconstitution

Dissolve in DMSO

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

ALEXA FLUOR is a trademark of Life Technologies

CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable