SAB4200648
Monoclonal Anti-SIRT1 antibody produced in mouse
clone SIRT1-1, purified from hybridoma cell culture
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About This Item
UNSPSC Code:
51111800
NACRES:
NA.41
clone:
SIRT1-1, monoclonal
application:
FACS
IP
IP
species reactivity:
human
technique(s):
flow cytometry: 5-10 μg/test using HeLa cells.
immunoblotting: 1-2 μg/mL using whole extracts of human Jurkat cells.
immunoprecipitation (IP): 5-10 μg using extracts of human Jurkat cells
immunoblotting: 1-2 μg/mL using whole extracts of human Jurkat cells.
immunoprecipitation (IP): 5-10 μg using extracts of human Jurkat cells
citations:
3
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
SIRT1-1, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
~1 mg/mL
technique(s)
flow cytometry: 5-10 μg/test using HeLa cells.
immunoblotting: 1-2 μg/mL using whole extracts of human Jurkat cells.
immunoprecipitation (IP): 5-10 μg using extracts of human Jurkat cells
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... Sirt1(23411)
General description
Monoclonal Anti-Sirt1 (mouse IgG2b isotype) is derived from the hybridoma SIRT1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human Sirt1 recombinant protein. Sirtuin 1 is a member of mammalian Sir2 gene family. It is present in the nucleus.
Immunogen
human Sirt1 recombinant protein
Application
Monoclonal Anti-SIRT1 antibody produced in mouse has been used in various immunochemical techniques including immunoblotting, immunoprecipitation and flow-cytometry.
Biochem/physiol Actions
Sirtuin 1 (Sirt1) is an NAD-dependent deacetylase and regulates various transcription factors, such as p53, FOX (forkhead box), nuclear factorΚB, myocyte enhancer factor-2 (Mef2) and peroxisome proliferator-activated receptor γ coactivator 1 α (PGC1α). Sirt1 deacetylates and downregulates p53. Increased activity of Sirt1 in culture cells reduces p53 transcriptional activity and p53-mediated apoptosis in response to radiation or oxidative stress. In addition, it also deacetylates and represses the activity of the forkhead transcription factor FOXO3a, other mammalian forkhead factors and reduces forkhead dependent apoptosis. Sirt1 plays a key role in myocyte differentiation by binding to the myoD cofactor, pCAF. It has been shown that Sirt1 modifies chromatin and silences transcription of integrated reporter genes via histone deacetylation. Sirt1 provides protection against neurodegeneration in models for Alzheimer′s disease and amyotrophic lateral sclerosis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Human SirT1 interacts with histone H1 and promotes formation of facultative heterochromatin
Vaquero A, et al.
Molecular Cell, 16(1), 93-105 (2004)
Marie Lagouge et al.
Cell, 127(6), 1109-1122 (2006-11-23)
Diminished mitochondrial oxidative phosphorylation and aerobic capacity are associated with reduced longevity. We tested whether resveratrol (RSV), which is known to extend lifespan, impacts mitochondrial function and metabolic homeostasis. Treatment of mice with RSV significantly increased their aerobic capacity, as
Hiroyasu Yamamoto et al.
Molecular endocrinology (Baltimore, Md.), 21(8), 1745-1755 (2007-04-26)
Sirtuins or Sir2 (silent information regulator 2)-related enzymes have originally been defined as a family of nicotinamide adenine dinucleotide-dependent enzymes that deacetylate lysine residue on various proteins. Certain sirtuins have in addition an ADP-ribosyltransferase activity. The sirtuins are remarkably conserved
William C Hallows et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(27), 10230-10235 (2006-06-23)
Silent Information Regulator 2 (Sir2) enzymes (or sirtuins) are NAD(+)-dependent deacetylases that modulate gene silencing, aging and energy metabolism. Previous work has implicated several transcription factors as sirtuin targets. Here, we investigated whether mammalian sirtuins could directly control the activity
Dohoon Kim et al.
The EMBO journal, 26(13), 3169-3179 (2007-06-22)
A progressive loss of neurons with age underlies a variety of debilitating neurological disorders, including Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS), yet few effective treatments are currently available. The SIR2 gene promotes longevity in a variety of organisms
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