SAB4200248

Anti-Actin antibody, Mouse monoclonal

clone MM2/193, purified from hybridoma cell culture

• MDL number: MFCD00145889
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: MM2/193, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~42 kDa
• species reactivity: mouse, human, rat, canine, monkey
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using HeLa cell extracts
• isotype: IgM
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTB(60)

Description

General description

Monoclonal anti-actin (mouse IgM isotype) is derived from the hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes. Monoclonal anti-actin recognizes human, monkey, dog, rat and mouse actin (not tested in other species).
In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms α_skeletal-actin, α _cardiac-actin, α_smooth-actin and γ_smooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.

Immunogen

hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes

Application

Monoclonal Anti-Actin antibody produced in mouse has been used in immunoblotting.

Biochem/physiol Actions

Actin plays a vital role in various cellular processes, such as cell division, migration, junction formation, chromatin remodeling, transcriptional regulation, vesicle trafficking and cell shape regulation. Polymorphism in the smooth muscle alpha-actin (ACTA2) is associated with the development of thoracic aortic aneurysms and dissections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

Store at -20°C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable