SAB4200135

Anti-FIP200 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody

• Synonym(s): Anti-200 kDa FAK family kinase-interacting protein, Anti-CC1, Anti-DRAGOU14, Anti-RB1-inducible coiled-coil 1, Anti-RB1CC1
• UNSPSC Code: 12352203
• NACRES: NA.41
• conjugate: unconjugated
• application: WB
• clone: polyclonal
• species reactivity: canine, mouse, bovine, monkey, rat, human
• citations: 17
• technique(s): western blot: 1-2 μg/mL using whole extracts of HEK-293 cells overexpressing human FIP200

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~200 kDa
• species reactivity: canine, mouse, bovine, monkey, rat, human
• concentration: ~1 mg/mL
• technique(s): western blot: 1-2 μg/mL using whole extracts of HEK-293 cells overexpressing human FIP200
• UniProt accession no.: Q8TDY2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RB1CC1(9821) ; mouse ... Rb1cc1(12421) ; rat ... Rb1cc1(312927)

Description

General description

FIP200 (focal adhesion kinase family interacting protein of 200 kDa), also known as RB1CC1 (retinoblastoma 1-inducible coiled-coil 1), is a multifunctional protein. It is mainly localized in the cytoplasm. The FIP200 gene is mapped to human chromosome 8q11.23.

Specificity

Anti-FIP200 (C-terminal) recognizes human FIP200.

Application

Anti-FIP200 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Focal adhesion kinase family interacting protein of 200 kDa (FIP200) interacts with UNC-51-like kinase (ULK 1) and is essential for autophagy. It also interacts with Pyk2 (proline-rich tyrosine kinase 2), FAK (focal adhesion kinase), TSC1 (TSC complex subunit 1), p53, apoptosis signal-regulating kinase 1 (ASK1) and TRAF2 (TNF receptor associated factor 2) and regulates cell growth, cell proliferation, cell survival, cell adhesion and cell migration. Deficiency in FIP200 is associated with embryonic death and cancer development.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable