SAB4200113

Anti-EDC4 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-Ge-1, Anti-HEDLS (Human enhancer of decapping, large subunit), Anti-RCD-8
• UNSPSC Code: 12352203
• NACRES: NA.41
• conjugate: unconjugated
• application: IP; WB
• clone: polyclonal
• species reactivity: human
• citations: 3
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of HEK-293 cells over expressing human EDC4; western blot: 0.5-1 μg/mL using lysates of HEK-293 cells over expressing human EDC4

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~150 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of HEK-293 cells over expressing human EDC4; western blot: 0.5-1 μg/mL using lysates of HEK-293 cells over expressing human EDC4
• UniProt accession no.: Q6P2E9
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EDC4(23644) ; mouse ... Edc4(234699) ; rat ... Edc4(361399)

Description

General description

Enhancer of mRNA decapping 4 (EDC4) contains an N-terminal WD40 repeat and a C-terminal domain, which interacts with decapping enzyme (Dcp2) and mediates EDC4 oligomerization and P-body localization.

The EDC4 gene is located on the human chromosome at 16q22.1.

Specificity

Anti-EDC4 (C-terminal) specifically recognizes human EDC4.

Application

Anti-EDC4 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Enhancer of mRNA decapping 4 (EDC4) associates with mRNA-decapping enzyme 2 (hDcp2) and stimulates hDcp2 activity in vitro as well as mediates the interaction between decapping enzymes (DCP1 and DCP2).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable