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Key Documents

P5119

Sigma-Aldrich

Phosphate buffered saline

liquid, pH 7.2 (25 °C)

Synonym(s):

PBS

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About This Item

MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate buffered saline, pH 7.2 (25 °C)

sterility

autoclaved

Quality Level

form

liquid

pH

7.2 (25 °C)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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General description

Phosphate buffered saline (PBS) is an isotonic buffer solution. This water-based salt solution is made up of disodium hydrogen phosphate, sodium chloride and, in some preparations, potassium chloride and potassium dihydrogen phosphate. PBS is generally used in biological research.

Application

It has also been used for the dilution of capture antibody during α-hemolysin (Hla) enzyme-linked immunosorbent assay (ELISA).
Phosphate buffered saline has been used in bis-benzimide accumulation assay.

Components

Sodium chloride, 145 mM (0.85%) in phosphate buffer, 150 mM

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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ELISA for Alpha-hemolysin (Hla) in Methicilin-resistant Staphylococcus aureus (MRSA)
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Yong N Li et al.
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Horizontal cells (HCs) are involved in establishing the center-surround receptive field organization of photoreceptor and bipolar cells. In many species, HCs respond differentially to colors and may play a role in color vision. An earlier study from our laboratory suggested

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