P3842

Phosphothreonine-BSA

2 mg/mL, buffered aqueous solution

• Synonym(s): Phosphothreonine Antibody Inhibitor
• MDL number: MFCD01094916
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• form: buffered aqueous solution
• Quality Level: 200
• concentration: 2 mg/mL
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Phosphothreonine-BSA is a Phosphothreonine antibody inhibitor which blocks the reactivity of anti-phosphothreonine antibody. Antibodies against phosphorylated amino acid can be used as a tool for identification, quantification and immunoaffinity isolation of activated cellular proteins. The product specifically blocks the reactivity of anti-phosphothreonine antibody but does not block anti-phosphoserine- or anti-phosphotyrosine specific antibodies.

Specificity

Working dilution is at least 10 μg/ml by the specific inhibition of the reactivity of Monoclonal Anti-Phosphothreonine (Sigma Product No. P 3555) in ELISA.

Application

Phosphothreonine-BSA can be used in various immunochemical assays like ELISA and immunoblotting for the specific inhibition of the reactivity of anti-phosphothreonine antibodies.

Phosphothreonine-BSA has been used in Pro-Q Diamond phosphoprotein gel staining method to detect phosphoamino acids.

Biochem/physiol Actions

O-phospho-L-threonine conjugated to bovine serum albumin. Phosphothreonine antibody inhibitor specifically blocks the reactivity of anti-phosphothreonine antibody. It does not block the activity of anti-phosphotyrosine or anti-phosphoserine-specific antibodies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable