L9017
Lectin from Phaseolus vulgaris (red kidney bean)
Phytohemagglutinin PHA-P, BioXtra, purified by affinity chromatography, lyophilized powder
Synonym(s):
Phaseolus vulgaris agglutinin, PHA
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About This Item
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biological source
Phaseolus vulgaris
Quality Level
product line
BioXtra
form
lyophilized powder
potency
≤17 μg per mL agglutination activity
purified by
affinity chromatography
impurities
salt, essentially free
storage temp.
2-8°C
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General description
Lectins are carbohydrate-binding proteins, that contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.
Application
Lectin from Phaseolus vulgaris (red kidney bean) has been used:
- for phytohemagglutinin (PHA) swelling assay
- for lymphocyte activation assay
- to evaluate cell-mediated immune response
Biochem/physiol Actions
Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It has anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin is associated with toxic allergens and hemagglutinins. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.
Quality
Mitogenic activity at ≤10 μg per mL.
Analysis Note
Agglutination activity is expressed in mg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8 after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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