L2897

LB Broth with agar (Lennox)

Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

• Synonym(s): Lennox broth, Luria Bertani Agar
• UNSPSC Code: 12352200
• NACRES: NA.85

Properties

• grade: for molecular biology
• Quality Level: 200
• sterility: non-sterile
• form: powder
• composition: Agar, 15 g/L ; NaCl, 5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• technique(s): microbiological culture: suitable
• application(s): food and beverages; microbiology
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.

Application

LB Broth with agar (Lennox) has been used for:

• the transformation of competent E. coli cells
• to cultivate Ecc-15 bacteria
• embedding scaffold containing tissue derived from induced pluripotent stem cells (iPSCs)
• transmission electron microscopy (TEM) and immunohistochemistry (IHC)

It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Features and Benefits

Lennox LB powder with agar provides:

• A budget-friendly alternative to pre-poured plates
• Easy scale-up using larger package sizes
• Standard formulation

Preparation Note

1. Suspend 35 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml sterile 1 M magnesium sulfate after autoclaving.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves