K0265

Anti-Potassium Channel K_2P4.1 (TRAAK) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• Synonym(s): Anti-KCNK4, Anti-TWIK-related arachidonic acid-stimulated K⁺ channel
• MDL number: MFCD09753858
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: human, rat
• technique(s): immunohistochemistry: suitable; western blot: 1:200 using HEK-K_2P4.1 transfected cells or rat cerebellum lysate
• UniProt accession no.: Q9NYG8
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... KCNK4(50801) ; rat ... Kcnk4(116489)

Description

General description

The gene K2P4.1, also referred to as KCNK4 (potassium two pore domain channel subfamily K member 4), encodes a protein belonging to the subfamily of K2P (two pore domain K⁺ channel) channels called TRAAK (TWIK-related arachidonic acid-stimulated K⁺ channel). The TRAAK channels are expressed selectively in the nervous system. TRAAK channel contains two protomers, each with two distinct pore domains forming a two-fold symmetric potassium channel.

Specificity

The K_2P4.1 antibody is directed against a highly conserved epitope in the intraracellular C-terminal region of the human K_2P4.1 channel.

Immunogen

peptide NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human K_2P4.1.

Biochem/physiol Actions

The gene KCNK4 (potassium two pore domain channel subfamily K member 4) encodes a potassium channel that is mechanosensitive and is regulated by polyunsaturated fatty acids and temperature. The structure and chemical composition of the lipid bilayer has a profound effect on the functioning of this K⁺ channel. It is found to regulate the noxious input threshold in dorsal root ganglia neurons.

Physical form

Lyophilized powder from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.025% sodium azide.

Reconstitution

Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein, such as BSA (1-3%).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable