I3146

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

• Synonym(s): Human ITS Liquid Media, Insulin-transferrin-sodium selenite
• UNSPSC Code: 12352205
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• product line: BioReagent
• form: liquid
• technique(s): cell culture | mammalian: suitable; single cell analysis: suitable
• impurities: endotoxin, tested
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:

• α mouse liver 12 (AML-12) cells
• rat fetal testis explants
• human dental pulp stem cells (DPSC)

Biochem/physiol Actions

• Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
• Transferrin: an iron-transport protein.
• Selenium: an essential trace element normally provided by serum.

Components

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.

Other Notes

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves