GE29327535

TempliPhi^™ 25 Amplification Sample Kit

Cytiva 29327535

• UNSPSC Code: 41106500

Properties

• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• manufacturer/tradename: Cytiva 29327535
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

illustra^™ TempliPhi^™DNA Amplification Kits use isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates.TempliPhi^™ Kits use a unique process to efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi^™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30° C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi^™ protocol requires less than 20 min of hands-on time to amplify 96 samples from bacterial colonies.Two different types of TempliPhi^™ DNA Amplification Kits are available to meet different throughput requirements. illustra^™ TempliPhi^™ DNA Amplification Kit for 2000 reactions contains premixed components and generates templates after overnight incubation. The 100- and 500-reaction kits contain separate, unmixed components for enhanced stability.The illustra^™ TempliPhi^™ DNA Sequencing Template Amplification Kit has a distinct protocol and workflow compared to the 100/500 reaction size. Procedures for each kit are not interchangeable. Prior optimization is needed.

Application

Preparation of circular DNA templates for cycle sequencing, cloning, and transformation.

Features and Benefits

• Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 h.
• Generate microgram quantities of template DNA from picogram amounts of starting material.
• Use amplified DNA directly for cycle sequencing without purification.Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA.
• Simple protocol reduces time, labor, and consumables needed for template preparation, and workflow enables easy automation.
• For low-to medium-throughput, small DNA vector preparation.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

TempliPhiOn packaging and insert for kits:For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,198,543, 5,576,204, and their equivalents in other countries.On container:Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and their equivalents in other countries.

TempliPhi is a trademark of Cytiva

illustra is a trademark of Cytiva

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids