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Key Documents

GE17-0456-01

Pharmalyte® 3 - 10

Cytiva 17-0456-01

Synonym(s):

carrier ampholytes, isoelectric focusing

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About This Item

UNSPSC Code:
41105323
NACRES:
NA.25

form

liquid

shelf life

3 yr

feature

wetted part: no

packaging

pkg of 25 mL

manufacturer/tradename

Cytiva 17-0456-01

storage condition

(S-re at 2°C - 8°C.)

color

colorless to light yellow
 Colorless to light yellow

solubility

cold water: soluble (Soluble in cold water and hot water)
soluble (in cold and hot water)

storage temp.

2-8°C

General description

Pharmalyte® carrier ampholytes form a stable linear pH gradient with even conductivity across the gel or in the capillary. Made by co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin. For isoelectric focusing (IEF) and IEF-PAGE quality control. Pharmalyte™ carrier ampholytes, prepared by the co-polymerization of glycine, glycylglycine, amines, and epichlorohydrin, are available in one broad-range and four narrow-range pH intervals. Each interval contains numerous ampholytes with a high buffering capacity per pH unit. Pharmalyte™ carrier ampholytes form an extremely stable linear pH gradient and exhibit even conductivity across the gel.

Application

Can be used directly for agarose, Ultrodex or polyacrylamide gels and form linear gradients.

Features and Benefits

  • High buffer capacity per pH unit
  • Linear and reproducible pH gradient
  • High resolution
  • Available in five broad-range and four narrow-range pH intervals

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Pharmalyte is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Components of Rehydration Solution

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Troubleshooting First-Dimension IEF: MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit

This page describes troubleshooting first-dimension isoelectric focusing using MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit.

Protocols

Protease Inhibition Using Protease Inhibitor Mix

Protease Inhibitor Mix from Cytiva inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.

Processing Small Tissue or Cell Samples using Sample Grinding Kit

Sample Grinding Kit from Cytiva is designed to disrupt cell or tissue samples for protein extraction.

Processing Small Tissue or Cell Samples using Sample Grinding Kit

Sample Grinding Kit from Cytiva Healthcare is designed to disrupt cell or tissue samples for protein extraction

Two-Dimensional Separation of Protein Samples

This page describes two-dimensional separation of protein samples with Cytiva products.

See All

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