GE17-0403-01
HiTrap® Protein A High Performance
Cytiva 17-0403-01, pack of 1 × 5 mL
About This Item
Recommended Products
ligand
native protein A
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 1 × 5 mL
manufacturer/tradename
Cytiva 17-0403-01
storage condition
(20% Ethanol)
parameter
flow rate
<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-10(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-9
storage temp.
2-8°C
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General description
HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.
Features and Benefits
- High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
- 34 μm bead size for high-performance, high-resolution purifications.
- Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System.
- Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
- Robust, easy-to-use method.
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
General instructions for affinity purification using HiTrap columns from Cytiva.
Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
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