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Key Documents

GE17-0403-01

HiTrap® Protein A High Performance

Cytiva 17-0403-01, pack of 1 × 5 mL

Synonym(s):

Protein A Affinity Column

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

native protein A

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 17-0403-01

storage condition

(20% Ethanol)

parameter

flow rate
<20 mL/min flow rate
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-10

working range

3-9

storage temp.

2-8°C

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General description

HiTrap® Protein A HP is a prepacked Protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap® Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Features and Benefits

  • High binding capacitiy of 20 mg IgG/mL medium and fast kinetics.
  • 34 μm bead size for high-performance, high-resolution purifications.
  • Designed for use with syringe, pump, or chromatography system such as AKTA design or FPLC™ System.
  • Stable over pH 3 to 9 for use, and 2 to 10 for cleaning.
  • Robust, easy-to-use method.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

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Certificates of Analysis (COA)

Lot/Batch Number

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