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F4055

Sigma-Aldrich

Anti-FMR1 (C-terminal) antibody produced in rabbit

enhanced validation

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-FMRP, Anti-Fragile X Mental Retardation Protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~80 kDa

species reactivity

human, mouse, rat

enhanced validation

functional assay
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using HEK-293T cells lysate
indirect immunofluorescence: 2-5 μg/mL using methanol-acetone fixed heat-shocked NIH3T3 cells
western blot: 1-2 μg/mL using HEK-293T cell lysate
western blot: 2-4 μg/mL using RAT1 cell lysate

UniProt accession no.

Q06787

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FMR1(2332)
mouse ... Fmr1(14265)
rat ... Fmr1(24948)

Related Categories

General description

FMR1 is a RNA binding protein expressed mainly in brain, neurons, placenta, testes and lymphocytes. Defect in FMR1 can lead to Fragile X Mental Retardation Syndrome due to lack of expression of FMR1 or expression of a mutant protein that cannot bind RNA. Anti-FMR1 (C-terminal) antibody can be used in immunofluorescence staining. Rabbit anti-FMR1 (C-terminal) antibody reacts specifically with FMR1.
The FMR1 protein (fragile X mental retardation 1 protein) can bind to RNA. It contains two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one RGG box. Two proteins named FXR1 and FXR2 interact with FMR1. The protein is highly expressed in brain and testis.

Immunogen

synthetic peptide corresponding to amino acids 606-623 of human FMR1, conjugated to KLH. The corresponding sequence is highly conserved (1 amino acid substitution) in rat and mouse.

Application

Anti-FMR1 (C-terminal) antibody produced in rabbit has been used in: western blotting, immunoprecipitation, immunofluorescence, immunoblotting .

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Giuseppe LaFauci et al.
Genes, 7(12) (2016-12-13)
The final product of FMR1 gene transcription, Fragile X Mental Retardation Protein 1 (FMRP), is an RNA binding protein that acts as a repressor of translation. FMRP is expressed in several tissues and plays important roles in neurogenesis, synaptic plasticity
Anita Torossian et al.
Neurobiology of disease, 148, 105213-105213 (2020-12-05)
SHANK3 is a postsynaptic scaffolding protein that plays a critical role in synaptic development and brain function. Mutations in SHANK3 are implicated in Phelan-McDermid syndrome (PMS), a neurodevelopmental disorder characterized by autistic-like behavior, delayed speech, hypotonia, and intellectual disability (ID).
Characterization of dFMR1, a Drosophila melanogaster homolog of the fragile X mental retardation protein
Wan L, et al.
Molecular and Cellular Biology, 20(22), 8536-8547 (2000)
Christina Gross et al.
Cell reports, 11(5), 727-736 (2015-04-30)
The PI3K enhancer PIKE links PI3K catalytic subunits to group 1 metabotropic glutamate receptors (mGlu1/5) and activates PI3K signaling. The roles of PIKE in synaptic plasticity and the etiology of mental disorders are unknown. Here, we show that increased PIKE
Differential Regulation of Syngap1 Translation by FMRP Modulates eEF2 Mediated Response on NMDAR Activity
Paul A, et al.
Frontiers in Molecular Neuroscience, 12 (2019)
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