F0895

FIBRONECTIN FROM HUMAN PLASMA

liquid, 0.1% (Solution), BioReagent, suitable for cell culture

• Synonym(s): Fibronectin
• MDL number: MFCD00131062
• UNSPSC Code: 12352202
• eCl@ss: 42020141
• NACRES: NA.75

Properties

• biological source: human plasma
• Quality Level: 200
• sterility: sterile
• product line: BioReagent
• form: liquid
• packaging: pkg of 1 mg; pkg of 2 mg; pkg of 5 mg
• concentration: 0.1% (Solution)
• technique(s): cell culture | mammalian: suitable
• surface coverage: 1‑5 μg/cm²
• solubility: saline: 0.05 M (Tris-buffered)
• UniProt accession no.: P02751
• Binding Specificity: Peptide Source: Collagen
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... FN1(2335)

Description

General description

Fibronectin 1 is a glycoprotein of the extracellular matrix that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. The FN1 gene mapped to human chromosome 2q35.
FN occurs as a dimeric protein, linked by two disulfide bonds at the end of C-terminal. Within the fibronectin structure, the domains are compactly arranged close to both the amino and carboxyl terminal of the peptide chains. Moreover, it has been observed that despite arising from a single gene up to 20 distinct variants of this gene have been identified in humans.

Application

Fibronectin from human plasma has been used in the determination of H₂O₂ consumption rate. It has also been used in coating the Sylgard^® models for cell culture. It has also been used in cell adhesion and to directly pattern the protein onto the polymeric film.

Biochem/physiol Actions

Fibronectin function encompasses a diverse range of biological processes including cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Fibronectin protein plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, hemostasis and thrombosis. Mutations in FN1 results in glomerulopathy.

Features and Benefits

• Adhesive glycoprotein
• Derived from human plasma
• Recommended surface coverage of 1-5 μg/cm2

Preparation Note

This product is a 0.1% solution of fibronectin. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C

Analysis Note

Protein homogeneity is evaluated by immunoelectrophoresis.

Legal Information

Sylgard is a registered trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable