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D8662

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
glucose: no (Dextro)

shipped in

ambient

storage temp.

2-8°C

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General description

Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.

Application

D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
  • as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
  • to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
  • to wash cells for protein isolation

Dulbecco′s Phosphate Buffered Saline has been used:
  • as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
  • to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
  • to wash cells for protein isolation

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
  • provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
  • serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
  • when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
  • provides a buffering system to maintain the medium within the physiological pH range

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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E Valas Teuma et al.
Journal of cataract and refractive surgery, 45(8), 1177-1182 (2019-07-06)
To compare the capsulotomy rim strength with capsular marks (CMs) to the rim strength without CMs in porcine eyes, and to demonstrate the practicality of CMs for intraoperative toric intraocular lens (IOL) alignment. LENSAR facility, Orlando, Florida, USA. Laboratory study.
Michele Russo et al.
European journal of heart failure, 21(4), 519-528 (2019-03-08)
Butyric acid (BUT), a short chain fatty acid produced daily by the gut microbiota, has proven beneficial in models of cardiovascular diseases. With advancements in cancer survival, an increasing number of patients are at risk of anticancer drug cardiotoxicity. Here
Ivan Kopljar et al.
British journal of pharmacology, 174(21), 3766-3779 (2017-01-18)
In the pharmaceutical industry risk assessments of chronic cardiac safety liabilities are mostly performed during late stages of preclinical drug development using in vivo animal models. Here, we explored the potential of human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) to
Kimberly R Long et al.
Molecular biology of the cell, 28(19), 2508-2517 (2017-07-20)
Cells lining the proximal tubule (PT) have unique membrane specializations that are required to maintain the high-capacity ion transport and endocytic functions of this nephron segment. PT cells in vivo acutely regulate ion transport in response to changes in glomerular
Martina Musutova et al.
Frontiers in endocrinology, 9, 616-616 (2018-11-06)
Metabolic impairments associated with obstructive sleep apnea syndrome (OSA) are linked to tissue hypoxia, however, the explanatory molecular and endocrine mechanisms remain unknown. Using gas-permeable cultureware, we studied the chronic effects of mild and severe hypoxia on free fatty acid
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