D5809
JumpStart™ AccuTaq™ LA DNA Polymerase
Hot-start high fidelity Taq enzyme, 10X buffer included
Synonym(s):
JumpStart® AccuTaq™ LA DNA Polymerase
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About This Item
UNSPSC Code:
41106314
NACRES:
NA.55
form
liquid
usage
sufficient for 125 reactions
sufficient for 500 reactions
feature
Long & Accurate PCR
dNTPs included: no
hotstart
concentration
2.5 unit/μL
technique(s)
PCR: suitable
color
colorless
input
purified DNA
suitability
suitable for PCR
shipped in
wet ice
storage temp.
−20°C
General description
JumpStart™ AccuTaq™ LA DNA Polymerase is a combination of Sigma′s high-fidelity blend, AccuTaq long and accurate (LA) DNA Polymerase, and the Taq neutralizing JumpStart Taq antibody. This specially formulated enzyme is the best choice for long-distance and high-fidelity PCR, multiplex PCR, and PCR amplification of targets with variable lengths, such as amplification of cDNA libraries. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot-start mechanism provides increased specificity and higher target yield in comparison to standard amplification.
Application
JumpStart™ AccuTaq™ LA DNA Polymerase has been used:
- in polymerase chain reaction (PCR) for the amplification of soil DNA samples
- in accurate long range PCR of histone H2A.Z in bacterial artificial chromosome (BACs)
- as a component of the PCR reaction mix for amplification of viral DNA through conventional PCR
Features and Benefits
- JumpStart AccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
- Up to 6.5× greater fidelity in comparison to Taq DNA polymerase making it the enzyme of choice for multiplex PCR
- Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA
- Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods
Packaging
Supplied with 10× reaction buffer.
Unit Definition
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
Other Notes
View more detailed information on Accutaq products at www.sigma-aldrich.com/hotstart.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
Jacobs is a registered trademark of Jacobs
JumpStart is a trademark of Sigma-Aldrich Co. LLC
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
Protocols
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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