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Key Documents

D4139

Sigma-Aldrich

N,N-Dimethyl-p-phenylenediamine dihydrochloride

suitable for peroxidase test, ≥99.0% (titration)

Synonym(s):

4-Amino-N,N-dimethylaniline dihydrochloride, DMPD · 2HCl, DMPPDA · 2HCl

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About This Item

Linear Formula:
(CH3)2NC6H4NH2 · 2HCl
CAS Number:
Molecular Weight:
209.12
Beilstein:
3913463
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

Quality Level

Assay

≥99.0% (titration)

form

powder

mp

222 °C (dec.) (lit.)

solubility

water: 50 mg/mL, clear

suitability

suitable for peroxidase test

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].Cl[H].CN(C)c1ccc(N)cc1

InChI

1S/C8H12N2.2ClH/c1-10(2)8-5-3-7(9)4-6-8;;/h3-6H,9H2,1-2H3;2*1H

InChI key

IAEDWDXMFDKWFU-UHFFFAOYSA-N

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Application

N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) has been used in the HID-AB (high iron diamine-alcian blue) staining of intestinal tract samples.

Biochem/physiol Actions

N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) is used for the determination of peroxidase. DMPD is converted into a red pigment of semiquinone nature. It is also used in the HID-AB (high iron diamine-alcian blue) staining procedures, which are used to differentiate between sialomucin and sulfomucin in the gastrointestinal tract.

Caution

Darkens readily when exposed to air.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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STRAUS W
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An HPLC micro-method with fluorescence detection has been developed to determine total vitamin C (vit C) and dehydroascorbic acid (DHA) concentrations in human plasma samples. This method is based on the rapid, specific reaction of DHA with dimethyl-o-phenylenediamine (DMPD) to

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