D1152

Dulbecco′s Modified Eagle′s Medium - high glucose

HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture

• Synonym(s): DME, DMEM
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: sodium pyruvate: no; NaHCO₃: no; phenol red: yes; HEPES: 25 mM; L-glutamine: yes; glucose: high
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Quantity

Formulated to contain 17.3 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable