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Eos-HL-60

96100920, human blood, Lymphoblastoid

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About This Item

UNSPSC Code:
41106514
biological source:
human blood
growth mode:
Suspension
karyotype:
Not specified
morphology:
Lymphoblastoid
products:
Not specified
receptors:
Not specified
relevant disease(s):
cancer

Product Name

Eos-HL-60, 96100920

biological source

human blood

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian promyelocytic leukaemia

Cell Line Description

Eos-HL-60 is a variant of the promyelocytic cell line HL60 established by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR profiling

Application

Study of production and function of human eosinophils, analysis of differentiation.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 13,14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17

Culture Medium

IDMEM + 2mM Glutamine + 0.5mM Butyric acid + 10% Foetal Bovine Serum (FBS); pH7.6; add Butyric acid to the medium just before use.

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

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