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product name
PORTHOS, 93122323
biological source
Porcine thyroid
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Thyroglobulin, plasminogen activator (u-PA)
receptors
Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Porcine thyroid epithelial
Cell Line Description
Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.
Application
Regulation of thyroid cell metabolism
Culture Medium
Click:RPMI (1:1) + 10μg/ml Insulin + 10mM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 4 cells/cm2 using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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