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Key Documents

RR1022

90102526, rat unknown/unspecified, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

RR1022, 90102526

biological source

rat unknown/unspecified

growth mode

Adherent

karyotype

2n = 42

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Rat Amsterdam Schmidt-Ruppin sarcoma

Cell Line Description

Derived from a Rous sarcoma virus (Schmidt-Ruppin strain) induced tumour in an inbred Amsterdam rat. The cells are highly tumorigenic in new-born rats and also chickens. There is some evidence that the cell line is latently infected with the virus.

Application

Tumourigenicity and virus studies: Rous sarcoma virus

Culture Medium

Medium 199 (HBSS) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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