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biological source
mouse lung
description
Malignant mesothelioma from mouse lung
growth mode
Adherent
karyotype
Not specified
morphology
Cells are stellate-shaped
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse malignant mesothelioma
Cell Line Description
Mice (BALB/c female 6-8 weeks) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0148
Application
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
Culture Medium
RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 10% FCS
Subculture Routine
Optimal split ratio 1:16 - 1:32 (seeding density 0.2 x104 cells/cm2).Cells incubated at 37oC with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
Other Notes
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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia
Certificates of Analysis (COA)
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