PNT1A (SERUM FREE)
7052901, human prostate, Not specified
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About This Item
UNSPSC Code:
41106514
product name
PNT1A (SERUM FREE), 07052901
biological source
human prostate
growth mode
Semi-adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human post pubertal normal, immortalised with SV40, serum-free
Cell Line Description
This cell line is a derivative of the normal human prostate cell line PNT1A (Sigma cat no. 95012614) adapted to grow in serum-free medium. PNT1A was established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem.
Culture Medium
Serum-free Stemline Keratinocyte Medium II (Sigma cat no. S0196) + Keratinocyte Medium Supplement (Sigma cat no. K3136). To freeze cells use 50:50 fresh:conditioned medium + 10% DMSO
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2; 5% CO2; 37 ºC. Mixture of attached and suspension cells. To harvest attached cells use gentle agitation. If cells do not detach, then try using 0.25% trypsin. A trypsin inhibitor must be used to inactivate trypsin. Seed contents of one ampoule into a 25 cm2 flask.
Other Notes
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Certificates of Analysis (COA)
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