C9284

Catalase−Agarose

pH 5.4(isoelectric point), ammonium sulfate suspension, enzyme from bovine liver

• Synonym(s): H₂O₂:H₂O₂ Oxidoreductase
• Enzyme Commission number: 1.11.1.6 (BRENDA, IUBMB)
• MDL number: MFCD00081483
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: enzyme from bovine liver
• description: optimum pH ~ 7.0
• form: ammonium sulfate suspension
• mol wt: tetramer ~250 kDa
• concentration: ≥800 units/mL
• extent of labeling: 60,000-120,000 units per g agarose (1900-3800 units per ml of agarose)
• matrix: 4% cross-linked beaded agarose
• pH: 5.4(isoelectric point)
• storage temp.: 2-8°C

Description

Application

Catalase agarose has been used in a study to advance the treatment of oxidative stress in neurodegenerative diseases. Catalase agarose has also been used in a study to investigate the growth of cyanobacterium on agar media in the presence of heterotrophic bacteria.

Catalases have a wide range of industrial applications. The enzyme is used for the removal of traces of (H₂O₂) that is added to milk and cheese during sterilization. It is used for the degradation of peroxide used in textile bleaching to prevent any problems in subsequent dyeing. Coupled with oxidases, the enzyme is used to prevent the inactivation of oxidases by the action of high concentration of peroxide. It is also used to prevent side reactions caused by (H₂O₂) that can degrade the target product.

Biochem/physiol Actions

Catalase activity is constant over the pH range of 4.0-8.5. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu²⁺ results in degradation of the catalase molecule.

Caution

Freezing of solutions is not recommended.

Unit Definition

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH 7, containing preservative

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves