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C6108

Sigma-Aldrich

Calpain 1 human

aqueous glycerol solution

Synonym(s):

Calcium-activated neutral protease 1

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About This Item

Enzyme Commission number:
3.4.22.52 (BRENDA, IUBMB)
MDL number:
MFCD00217575
UNSPSC Code:
12352204
NACRES:
NA.54

Assay

≥95% (SDS-PAGE)

form

aqueous glycerol solution

specific activity

≥1000 U/mg

UniProt accession no.

P07384

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CAPN1(823)

Application

Human calpain 1 has been used in a study to assess how the crystal structures of human calpains 1 and 9 imply diverse mechanisms of action and auto-inhibition. Human calpain 1 has also been used in a study to investigate the synthesis, biological evaluation and molecular modelling of N-heterocyclic dipeptide aldehydes as selective calpain inhibitors.

Biochem/physiol Actions

Caplain 1 is a neutral calcium-dependent cysteine protease containing the EF-hand motif. The protease consists of two subunits; the larger subunit has four domains that are homologous with papain and calmodulin. The smaller subunit has one domain that shares homology with calmodulin. It is activated by micromolar levels of calcium and hence, it is also called as micro-calpain. Its activation leads to cellular protein degradation, neuronal cell degeneration, and autoimmune demyelinating diseases such as multiple sclerosis.
Cytosolic protease with involvement in cytoskeletal remodeling, autophagy, and apoptosis as an upstream regulator.

Physical properties

Calpain 1 is a heterodimeric calcium-activated thiol-protease.

Unit Definition

One unit will hydrolyze 1 picomole Suc-LLVY-AMC per minute at 25 deg C.

Physical form

Solution in 20 mM Imidazole-HCl, 5 mM β-Mercaptoethanol, 1 mM EDTA, 1 mM EGTA, and 30% glycerol.

inhibitor

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xuan Zhang et al.
PloS one, 7(2), e31458-e31458 (2012-02-23)
Hepatitis B virus X protein (HBx) plays an important role in the development of hepatocellular carcinoma (HCC). However, the mechanism remains unclear. Recently, we have reported that HBx promotes hepatoma cell migration through the upregulation of calpain small subunit 1
Matthew A Jones et al.
Bioorganic & medicinal chemistry, 16(14), 6911-6923 (2008-06-24)
A series of N-heterocyclic dipeptide aldehydes 4-13 have been synthesised and evaluated as inhibitors of ovine calpain 1 (o-CAPN1) and ovine calpain 2 (o-CAPN2). 5-Formyl-pyrrole 9 (IC(50) values of 290 and 25nM against o-CAPN1 and o-CAPN2, respectively) was the most
M K Squier et al.
Journal of immunology (Baltimore, Md. : 1950), 158(8), 3690-3697 (1997-04-15)
Apoptosis is the common phenotype of programmed or physiologic cell death, the process used to remove excess or defunct cells during normal tissue maintenance. One of the most studied cell types with respect to apoptosis is the immature T cell
Rebecca M Kandell et al.
ACS nano, 15(12), 20504-20516 (2021-12-07)
Traumatic brain injury (TBI) is a critical public health concern and major contributor to death and long-term disability. After the initial trauma, a sustained secondary injury involving a complex continuum of pathophysiology unfolds, ultimately leading to the destruction of nervous
R Zolfaghari et al.
The Biochemical journal, 241(1), 129-135 (1987-01-01)
A high-Mr neutral endopeptidase-24.5 (NE) that cleaved bradykinin at the Phe5-Ser6 bond was purified to apparent homogeneity from human lung by (NH4)2SO4 fractionation, ion-exchange chromatography and gel filtration. The final enzyme preparation produced a single enzymically active protein band after
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