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A8412

Sigma-Aldrich

Bovine Serum Albumin solution

7.5% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
9048-46-8
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.75

biological source

bovine

Quality Level

200

description

low endotoxin

sterility

sterile-filtered

product line

BioXtra

Assay

7.0-8.0% protein basis (UV)

form

liquid

mol wt

66.4 kDa

purified by

cold ethanol and heatshock fractionation

origin

USA origin

concentration

7.5% in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

≤13.0 EU/mL Endotoxin level

pH

6.5-7.5

foreign activity

Mycoplasma test (Negative)
cell growth >50%

shipped in

ambient

storage temp.

2-8°C

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General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Select the right albumin for your application with the Albumin Selection Guide.
Qualified for use in cell culture applications.

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media (SFM). It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

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Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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John R Horton et al.
Journal of medicinal chemistry, 61(7), 3193-3208 (2018-03-15)
Isomers of chiral drugs can exhibit marked differences in biological activities. We studied the binding and inhibitory activities of 12 compounds against KDM5A. Among them are two pairs of enantiomers representing two distinct inhibitor chemotypes, namely, ( R)- and (
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To evaluate the expression of mammalian target of rapamycin (mTOR) pathway molecules in mouse spermatogenesis and as well as its role during proliferation and meiotic initiation of spermatogenic cells. Experimental animal study. University. C57Balb-C adult male mice. Expressions of mTOR
William C Hines et al.
Breast cancer research and treatment, 155(1), 37-52 (2015-12-15)
Despite the prevalence and significant morbidity resulting from estrogen receptor positive (ER(+)) breast adenocarcinomas, there are only a few models of this cancer subtype available for drug development and arguably none for studying etiology. Those models that do exist have
Amanda J Shapiro et al.
Veterinary microbiology, 177(1-2), 154-161 (2015-03-18)
The seroprevalence of Coxiella burnetii (C. burnetii) in cats in eastern Australia is unknown, and the risk of transmission from cats to humans is undetermined. This study aimed to determine the exposure of cats to C. burnetii in four distinct
Paul J Orchard et al.
Scientific reports, 9(1), 7858-7858 (2019-05-28)
Cerebral adrenoleukodystrophy (cALD) is an inflammatory neurodegenerative disease associated with mutation of the ABCD1 gene. Proteomic analysis of cerebral spinal fluid (CSF) from young males with active cALD revealed markers of inflammation including APOE4. APOE4 genotype has been associated with
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Articles

Albumin in Cell Culture

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

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