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Key Documents

A6926

Sigma-Aldrich

3-Amino-9-ethylcarbazole

tablet

Synonym(s):

9-Ethylcarbazol-3-amine, AEC

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About This Item

Empirical Formula (Hill Notation):
C14H14N2
CAS Number:
Molecular Weight:
210.27
Beilstein:
174969
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83

form

tablet

Quality Level

solubility

DMF: 1 tablet/5 mL

SMILES string

CCn1c2ccccc2c3cc(N)ccc13

InChI

1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3

InChI key

OXEUETBFKVCRNP-UHFFFAOYSA-N

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General description

3-Amino-9-ethylcarbazole is a chromogen

Specificity

3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.

Application

3-Amino-9-ethylcarbazole (AEC) has been used as a chromogen in immunohistochemistry to visualize the cells. It has also been used as a substrate in enzyme-linked immune absorbent spot (ELISpot).

Quantity

Contains 20 mg substrate per tablet.

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Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 3 Oral - Carc. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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