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74385

Sigma-Aldrich

Nonidet™ P 40 Substitute

BioXtra, mixture of 15 homologues

Synonym(s):

4-Nonylphenyl-polyethylene glycol, Imbentin-N/52, NP 40

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About This Item

CAS Number:
9016-45-9
MDL number:
MFCD00132851
UNSPSC Code:
12161900
NACRES:
NA.25

description

TLC: corresponds to standard
non-ionic

product line

BioXtra

form

liquid

mol wt

average mol wt 680

refractive index

n20/D 1.49

pH

5-8 (5% in H2O)

CMC

0.059 mM (20-25°C)

transition temp

cloud point 45-50 °C

density

1.06 g/mL at 20 °C

anion traces

chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤1000 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

HLB

13.5

InChI

1S/C19H32O3/c1-2-3-4-5-6-7-8-9-18-10-12-19(13-11-18)22-17-16-21-15-14-20/h10-13,20H,2-9,14-17H2,1H3

InChI key

BLXVTZPGEOGTGG-UHFFFAOYSA-N

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Application

Non-ionic surfactant, used to solubilize cerebral GABA receptors. Used in solubilization of proteins and lipids of the scallop gill ciliary membrane and of membranes from breast cancer tumors.

Caution

May become turbid on storage; a clear product can be obtained on heating to 40°C.

Legal Information

Nonidet is a trademark of Shell Chemical Co.

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD4037
BCCC9108
BCCB9285
BCCB0820
BCCB0819

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R E Stephens
The Journal of cell biology, 100(4), 1082-1090 (1985-04-01)
The proteins and lipids of the scallop gill ciliary membrane may be reassociated through several cycles of detergent solubilization, detergent removal, and freeze-thaw, without significant change in overall protein composition. Membrane proteins and lipids reassociate to form vesicles of uniform
B Schraag et al.
Analytical biochemistry, 211(2), 233-239 (1993-06-01)
A procedure for an enzyme-linked immunosorbent assay for the determination of protein tyrosine kinase (PTK) activity from cytosolic and solubilized membrane fractions from breast cancers, is described. The general PTK substrate poly(GluNa, Tyr) 4:1 is coated to the wells of
Ito, Y.
Int. Congr. Ser. - Excerpta Med., 565, 31633-31633 (1982)
Christopher Gallagher et al.
Scientific reports, 7(1), 14083-14083 (2017-10-28)
Most eukaryotic RNA regulators recognise their RNA and protein partners by the combinatorial use of several RNA binding domains. Inter-domain dynamics and interactions play a key role in recognition and can be analysed by techniques such as NMR or FRET
Elise Malek-Adamian et al.
Nucleic acid therapeutics, 29(4), 187-194 (2019-05-16)
In this study, we explore the effect of a library of 2'-, 4'-, and 2',4'-modified uridine nucleosides and their impact on silencing firefly luciferase and on down-regulated in renal cell carcinoma (DRR) gene targets. The modifications studied were 2'-F-ribose, 2'-F-arabinose
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