18470

Bromothymol Blue sodium salt

for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6)

• Synonym(s): Bromthymol Blue sodium salt, 3′,3′′-dibromothymolsulfonephthalein sodium salt
• Empirical Formula (Hill Notation): C₂₇H₂₇Br₂NaO₅S
• CAS Number: 34722-90-2
• Molecular Weight: 646.36
• Beilstein: 6887926
• EC Number: 252-169-7
• MDL number: MFCD00077263
• UNSPSC Code: 12352200
• PubChem Substance ID: 57647742
• NACRES: NA.25

Properties

• grade: for microscopy (Bot., Hist., Vit.); for molecular biology; indicator (pH 6.0-7.6)
• Quality Level: 100
• form: powder
• technique(s): titration: suitable
• visual transition interval: 6.0-7.6, yellow to blue
• storage temp.: room temp
• SMILES string: [Na+].CC(C)c1cc(c(C)c(Br)c1O)C(=C2\C=C(C(C)C)C(=O)C(Br)=C2C)\c3ccccc3S([O-])(=O)=O
• InChI: 1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;
• InChI key: NMKFVGALBGZKGW-FKWCIMQXSA-M

Description

General description

Bromothymol blue is a pH indicator for weak acids and bases. In neutral solution, it appears bluish-green. At pH above 7.6 it appears blue, and at pH below 6.0 it appears yellow.
Bromothymol blue (BTB) is an anionic dye. It can be protonated or deprotonated to generate yellow or blue. BTB helps to make ion-pair complex, that is used to identify several pharmaceutical compounds with the help of extractive spectrophotometric methods.

Application

Suitable for use:

• In preparations of pH indicator solutions
• In phospholipid staining on tissues and cells prepared on microscopy slides

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves