05482
Bovine Serum Albumin
fraction V, ≥96% (GE)
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
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biological source
bovine
Assay
≥96% (GE)
form
lyophilized powder
mol wt
~66 kDa
Mr ~67000
purified by
heat shock fractionation
loss
≤5% loss on drying
pH
5.0-6.0 (10 mg/mL in H2O)
solubility
H2O: 40 mg/mL, clear to faintly turbid, faintly yellow to greenish-yellow
H2O: slightly soluble
storage temp.
2-8°C
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Application
Albumin from bovine serum has been used as a test compound for detecting the weaker, distal chromatin co-associations by using enhanced 4C (e4C, enhanced chromosome conformation capture on chip) technique.
Biochem/physiol Actions
Bovine serum albumin also referred to as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated as well as non-treated microwells. It can also trigger insulin dependent diabetes mellitus in humans.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
By a modified Cohn procedure, using cold ethanol, pH and low temperature precipitation.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Other Notes
As chiral complexing agent in the resolution of dansyl DL amino acids
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Certificates of Analysis (COA)
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Tom Sexton et al.
Nature protocols, 7(7), 1335-1350 (2012-06-23)
Chromosome conformation capture (3C) is a powerful technique for analyzing spatial chromatin organization in vivo. Technical variants of the assay ('4C') allow the systematic detection of genome-wide coassociations with bait sequences of interest, enabling the nuclear environments of specific genes
L. Lepri et al.
Chromatographia, 36, 297-297 (1993)
J Karjalainen et al.
The New England journal of medicine, 327(5), 302-307 (1992-07-30)
Cow's milk has been implicated as a possible trigger of the autoimmune response that destroys pancreatic beta cells in genetically susceptible hosts, thus causing diabetes mellitus. Studies in animals have suggested that bovine serum albumin (BSA) is the milk protein
M Steinitz
Analytical biochemistry, 282(2), 232-238 (2000-06-30)
ELISA provides a highly sensitive procedure for quantitating antigens and antibodies. In that assay, microwells are coated initially with a specific ligand and then saturated with inert molecules to minimize nonspecific background. Coating can be improved by pretreating the microwells
Preparation and properties of serum and plasma proteins; a system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids.
E J COHN et al.
Journal of the American Chemical Society, 68, 459-475 (1946-03-01)
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