TR-1008
Sodium Butyrate Solution
This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.
Synonym(s):
Cell Culture NaBu
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75
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Quality Level
form
liquid
manufacturer/tradename
Specialty Media
input
sample type: mouse embryonic stem cell(s)
General description
This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells. Available as a 500mM stock solution (1mL). A complete protocol is included with shipment.
Application
Sodium butyrate can act to increase the efficiency of transfection and expression for both transient and stable transfections. It increases enhancer-dependant transcription of inserts driven by the SV40 early promoter. Vectors of this type include any of the pSV and pSV2 plasmids. Treatment of transfected cells with sodium butyrate can result in: a 2-3 fold increase in the number of cells able to express the insert; a 10-30 fold increase in the number of stable transfected colonies; and cells treated with sodium butyrate at the time of transfection can be re-induced by a second treatment weeks after the initial treatment and yield a 4-7 fold increase in transcription of the SV40 early promoter driven insert.
This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.
This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.
Storage and Stability
Store at 2-8°C for 2 years.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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