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Sigma-Aldrich

NDiff Neuro-2 Medium Supplement (200x)

NDiff Neuro-2 Medium Supplement is a serum-free, N2-like supplement for the in vitro differentiation of murine embryonic stem (ES) cells into post-mitotic neurons particularly via monolayer differentiation.

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

100

form

liquid

manufacturer/tradename

Millipore Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

dry ice

General description

Originally developed in Professor Austin Smith′s laboratory, NDiff Neuro-2 Medium Supplement is a serum-free, N2-like supplement for the in vitro differentiation of murine embryonic stem (ES) cells into post-mitotic neurons particularly via monolayer differentiation. This product may also be used in the derivation, propagation and maintenance of mouse NS neural stem cells.

Physical form

NDiff Neuro-2 Medium Supplement contains Insulin, Apo-Transferrin, Sodium Selenite, Putrescine, and Progesterone. This media supplement is supplied as a 200X stock and should be added to the cell culture medium at a final concentration of 1X.

Storage and Stability

Maintain at -20 °C until expiration date. Protect from light. Once thawed, aliquot unused portion into smaller volumes and store at -20 °C until future use. Once added to the cell culture medium, the product is stable at 4 °C for four weeks.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Niche-independent symmetrical self-renewal of a mammalian tissue stem cell.
Conti, Luciano, et al.
PLoS Biology, 3, e283-e283 (2005)
Neural stem cell systems: diversities and properties after transplantation in animal models of diseases
Conti, Luciano, et al
Brain Pathology, 16, 143-154 (2006)
Adherent neural stem (NS) cells from fetal and adult forebrain
Pollard, Steven M, et al
Cerebral Cortex, 16 Suppl 1, i112-i120 (2006)
Optimization and integration of expansion and neural commitment of mouse embryonic stem cells.
Diogo, Maria Margarida, et al.
Biotechnology and Applied Biochemistry, 49, 105-112 (2008)
Dynamic cell-cell interactions between cord blood haematopoietic progenitors and the cellular niche are essential for the expansion of CD34+, CD34+CD38- and early lymphoid CD7+ cells.
da Silva, Claudia Lobato, et al.
Journal of Tissue Engineering and Regenerative Medicine, 4, 149-158 (2010)
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