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MCHT12H48

Millipore

Millicell® Hanging Cell Culture Insert, PET 0.4 μm, 12-well, 48/pk

Discontinued with Replacement Product No. PTHT12H48

Synonym(s):

cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate

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About This Item

UNSPSC Code:
41121800

material

PET membrane
flat wells
polystyrene housing

sterility

sterile

feature

lid: no

packaging

pack of 48 ea (individually blister packaged)

parameter

50 °C max. temp.

filter diam.

12 mm

filtration area

1.1 cm2

size

12 wells

surface area

1.1 cm2

well maximum volume

1.4 mL

working volume

400 μL

pore size

0.4 μm

input

sample type mesenchymal stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type cardiac stem cell(s)
sample type pancreatic stem cell(s)
sample type: human embryonic stem cell(s)

binding type

Tissue Culture (TC)-treated surface

General description

Polyethylene Terephthalate (PET) hanging cell culture insert with pore size of 8.0 μm used in a 24-well plate for cell attachment, cell culture, cell differentiation & ICC.Device configuration: 12-well plate, 0.4 μm pore sizeMillicell® cell culture inserts are an easy to use and convenient device for adherent or suspension cells. Allowing the access of media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.
Millicell® Hanging Inserts: Preloaded in receivers or available standalone
  • For co-culturing and permeability assays
  • Unique design allows easier basolateral access than other hanging inserts with less risk of contamination
  • Available in 5 pore sizes and 3 diameters, including a 1 μm pore size that is optically transparent for better visualization by microscopy

Applications: Cell Attachment, Cell Growth, Cell Differentiation, Immunocytochemistry

Application

Polyethylene Terephthalate hanging cell culture insert with pore size of 0.4 μm used in a 12-well plate for cell attachment, cell culture, cell differentiation & ICC.

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

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16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.

Protocols

Generating organotypic 3D skin cultures as an in-vitro model of the human epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

Microscopic Examination of Samples

This protocol covers three methods for the microscopic examination of cell samples.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This technical article covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

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