biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies, primary antibodies
clone
3F11, monoclonal
mol wt
calculated mol wt 84.33 kDa
observed mol wt ~120 kDa
purified by
using protein G
species reactivity
mouse, human, rat, canine, porcine
packaging
antibody small pack of 100 μg
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
Extracellular domain
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... FOLH1(2346)
General description
Glutamate carboxypeptidase 2 (UniProt: Q04609; also known as EC:3.4.17.21, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Membrane glutamate carboxypeptidase, mGCP, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate-specific membrane antigen, PSM, PSMA, Pteroylpoly-gamma-glutamate carboxypeptidase) is encoded by the FOLH1 (also known as FOLH, NAALADI, PSM, PSMA, GIG27) gene (Gene ID: 2346) in human. PSMA is a single-pass type II membrane protein that has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase activities. It exhibits a preference for tri-alpha-glutamate peptides. Its expression pattern is restricted mainly to the healthy prostate secretory-acinar epithelium and the plasma membrane of epithelial prostate carcinoma. In the brain, it modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG) and release of glutamate. Clone 3F11 is shown to be highly specific for PSMA and does not exhibit any cross-reactivity with unrelated proteins. It binds to a linear epitope (aa 226-243) within the extracellular domain and is reported to be suitable for the detection of PSMA orthologs from some other species when denatured form of PSMA is used. (Ref.: Vahidfar, N., et al., (2019). J. Radioanal. Nucl. Chem. 322:237 248; Novakova, Z., et al. (2017). The Prostate 77(7); 749-764).
Specificity
Clone 3F11 is a mouse monoclonal antibody that detects Prostate-specific membrane antigen (PSMA). It targets an epitope within 18 amino acids from the extracellular domain.
Immunogen
Full-length recombinant human Prostate-specific membrane antigen (PSMA).
Application
Quality Control Testing
Evaluated by Western Blotting in LNCap cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PSMA in LNCap cell lysate.
Tested Applications
Immunofluorescence Analysis: A representative lot detected PSMA in Immunofluorescence applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).
ELISA Analysis: A representative lot detected PSMA in ELISA applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).
Immunohistochemistry (Paraffin) Analysis: A 1:80,000 dilution from a representative lot detected PSMA in human prostate tissues (Courtesy of Barinka, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic and J. Hrudka, University Hospital Vinohrady, Prague, Czech Republic).
Immunohistochemistry Applications: A representative lot detected PSMA in Immunohistochemistry applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).
Immunocytochemistry Analysis: A representative lot detected PSMA in Immunocytochemistry applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).
Western Blotting Analysis: A representative lot detected PSMA in Western Blotting applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in LNCap cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PSMA in LNCap cell lysate.
Tested Applications
Immunofluorescence Analysis: A representative lot detected PSMA in Immunofluorescence applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).
ELISA Analysis: A representative lot detected PSMA in ELISA applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).
Immunohistochemistry (Paraffin) Analysis: A 1:80,000 dilution from a representative lot detected PSMA in human prostate tissues (Courtesy of Barinka, Institute of Biotechnology of the Czech Academy of Sciences, Vestec, Czech Republic and J. Hrudka, University Hospital Vinohrady, Prague, Czech Republic).
Immunohistochemistry Applications: A representative lot detected PSMA in Immunohistochemistry applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).
Immunocytochemistry Analysis: A representative lot detected PSMA in Immunocytochemistry applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).
Western Blotting Analysis: A representative lot detected PSMA in Western Blotting applications (Novakova, Z., et al. (2017). Prostate. 77(7):749-764).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PSMA/FOLH1, clone 3F11, Cat. No. MABN2300, is a mouse monoclonal antibody that detects Prostate-specific membrane antigen (PSMA) and is tested in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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