MABE241
Anti-dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12
ascites fluid, clone 51TA-2H1, from mouse
Synonym(s):
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
51TA-2H1, monoclonal
species reactivity
human
technique(s)
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer10/pSer28), dimethylation (Lys9/Lys27)
Gene Information
human ... HIST1H3F(8968)
General description
Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Methylation occurs on histone H3 at lysine 4, 9, 27, 36, and 79 residues. Previous studies have reported that methylation of H3 lysine 9 provides a binding site for heterochromatin adaptor proteins (HP1) which are involved in the formation of heterochromatin and gene repression. Conversely,deacetylation of histone H3 lysine 9 is associated with gene-activating events. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
Specificity
This antibody recognizes Histone H3 dimethylated and phosphorylated at Lys9/27 and Ser10/28.
Immunogen
Epitope: Dimethylated and phosphorylated at Lys9/27 and Ser10/28.
Ovalbumin-conjugated linear peptide corresponding to human Histone H3 dimethylated and phosphorylated at Lys9/27 and Ser10/28.
Application
Anti-dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) Antibody, clone 51TA-2H12 is a Mouse Monoclonal Antibody for detection of dimethyl-phospho Histone H3 (Lys9/27)/(Ser10/28) also known as Histone H3.1 & has been validated in WB, DB, ICC, IP & IHC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blot Analysis: A representative lot was used by an independent laboratory in KCl whole-cell extract. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Dot Blot Analysis: A representative lot was used by an independent laboratory in unmodified and modified Histone H3 peptides. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in NIH/3T3 cells. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in mouse testis tissue. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Dot Blot Analysis: A representative lot was used by an independent laboratory in unmodified and modified Histone H3 peptides. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in NIH/3T3 cells. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in mouse testis tissue. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Eberlin, A., et al. (2008). Molecular and Cellular Biology. 28(5):1739–1754.)
Quality
Evaluated by Western Blot in untreated and etoposide acid extract treated HeLa cell lysates.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected Histone H3 on 10 µg of untreated and etoposide acid extract treated HeLa cell lysates.
Western Blot Analysis: A 1:5,000 dilution of this antibody detected Histone H3 on 10 µg of untreated and etoposide acid extract treated HeLa cell lysates.
Target description
~17 kDa observed
Physical form
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Untreated and etoposide acid extract treated HeLa cell lysates.
Untreated and etoposide acid extract treated HeLa cell lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Histone H3 tails containing dimethylated lysine and adjacent phosphorylated serine modifications adopt a specific conformation during mitosis and meiosis.
Eberlin, Adrien, et al.
Molecular and cellular biology, 28, 1739-1754 (2008)
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