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MAB4417

Sigma-Aldrich

Anti-Achaete Scute homolog 2 Antibody, clone 8F1

clone 8F1, from mouse

Synonym(s):

achaete-scute complex (Drosophila) homolog-like 2, achaete-scute complex homolog 2 (Drosophila), achaete-scute complex homolog-like 2, achaete-scute complex-like 2, achaete-scute complex-like 2 (Drosophila), mammalian achaete/scute homologue 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8F1, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_032580.2

UniProt accession no.

O35885

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ASCL2(430)

General description

Achaete Scute homolog 2 (also known as mammalian aschaete-scute homolog 2 or Mash2) is a nuclear protein expressed specifically in the extravillous trophoblasts of developing placenta. Placental development involves control by the basic helix-loop-helix transcription factor Achaete Scute homolog 2, which may regulate HIF (hypoxia) in the formation of spongiotrophoblasts. Targeted mutagenesis of Achaete Scute homolog 2 yielded loss of function results in embryonic lethality at midgestation due to placental failure associated with a lack of spongiotrophoblasts and reduced labyrinthine trophoblast layers. In addition to healthy placental development, Achaete Scute proteins may also be involved in the determination of neuronal precursors in the central and peripheral nervous systems. Achaete Scute homolog 2 has also been implicated as a controller of intestinal stem cell fate and is essential for the maintenance of adult intestinal stem cells (Tanaka, M., et al., (1997) Dev Biol. 190(10):55-65).

Specificity

This antibody recognizes Achaete Scute homolog 2.

Immunogen

His-tagged recombinant protein corresponding to mouse Achaete Scute homolog 2.

Application

Anti-Achaete Scute homolog 2 Antibody, clone 8F1 is an antibody against Achaete Scute homolog 2 for use in WB, IH.
Immunohistochemistry: A previous lot of this antibody was used in immunocytochemistry by an independent laboratory (Van der Flier, et al., 2009).

Quality

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Achaete Scute homolog 2 on 10 µg of human placenta tissue lysate.

Target description

~ 20 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Qingyu Wang et al.
Stem cell reports, 15(2), 374-388 (2020-07-11)
Intestinal regeneration is crucial for functional restoration after injury, and nutritional molecules can play an important role in this process. Here, we found that arachidonic acid (AA) serves as a direct proliferation promoter of intestinal epithelial cells that facilitates small
Transcription factor achaete scute-like 2 controls intestinal stem cell fate.
van der Flier, LG; van Gijn, ME; Hatzis, P; Kujala, P; Haegebarth, A; Stange, DE; Begthel et al.
Cell null
Rosemary Oh-McGinnis et al.
Developmental biology, 351(2), 277-286 (2011-01-18)
Several imprinted genes have been implicated in the regulation of placental function and embryonic growth. On distal mouse chromosome 7, two clusters of imprinted genes, each regulated by its own imprinting center (IC), are separated by a poorly characterized region
Kevin C Allan et al.
Cell stem cell, 28(2), 257-272 (2020-10-23)
Mammalian cells respond to insufficient oxygen through transcriptional regulators called hypoxia-inducible factors (HIFs). Although transiently protective, prolonged HIF activity drives distinct pathological responses in different tissues. Using a model of chronic HIF1a accumulation in pluripotent-stem-cell-derived oligodendrocyte progenitors (OPCs), we demonstrate

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