MAB349

Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5

clone 1.D5, Chemicon^®, from mouse

• Synonym(s): Alz-90
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1.D5, monoclonal
• species reactivity: human, rat
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000484.2; NM_201413.1; NM_201414.1
• UniProt accession no.: P05067
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... APP(351)

Description

Specificity

Reacts with the natural amyloid precursor protein A4(695).

Immunogen

Epitope: A4(695)

Synthetic peptide Alz 90 [amino acids 511-608 of amyloid precursor protein pre A4(695)].

Application

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5 is validated for use in WB for the detection of Amyloid Precursor Protein.

Western blot: 10-20 μg/mL

Optimal working dilutions must be determined by the end user.

Westen Blot Protocol for MAB349

1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.

2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.

3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.

4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.

5) Repeat step #3.

6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.

7) Repeat step #3.

8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.

9) Repeat step #3.

Physical form

Format: Purified

Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.

Storage and Stability

Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable