MAB326
Anti-CNPase Antibody, clone 11-5B
clone 11-5B, Chemicon®, from mouse
Synonym(s):
2′3′-Cyclic Nucleotide 3′-Phosphohydrolase
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
11-5B, monoclonal
species reactivity
canine, sheep, mouse, pig, bovine, rat, rabbit, human
should not react with
guinea pig, chicken
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CNP(1267)
General description
CNPase (2′, 3′-cyclic nucleotide 3′-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
Specificity
CNPase, 48 and 46 kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc.
Immunogen
Purified human brain CNPase
Application
Detect CNPase using this Anti-CNPase Antibody, clone 11-5B validated for use in IC, IH, IH(P) & WB.
Immunocytochemistry:
A previous lot was used on primary oligodendrocyte cultures.
Immunohistochemistry:
A previous lot was used on rat hippocampus tissue and rat spinal cord.
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0).
Immunoblot:
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)
Optimal working dilutions must be determined by end user.
A previous lot was used on primary oligodendrocyte cultures.
Immunohistochemistry:
A previous lot was used on rat hippocampus tissue and rat spinal cord.
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0).
Immunoblot:
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Quality
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CNPASE on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CNPASE on 10 μg of Mouse Brain lysates.
Target description
48 & 46 kDa
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide
Storage and Stability
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Control
Western Blot: Fresh bovine whole brain extract, mouse brain lysate.
Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue.
Western Blot: Fresh bovine whole brain extract, mouse brain lysate.
Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Electroconvulsive seizures induce proliferation of NG2-expressing glial cells in adult rat amygdala.
Malin Wennstrom, Johan Hellsten, Anders Tingstrom
Biological Psychiatry null
Hereditary cerebellar degenerative disease (cerebellar cortical abiotrophy) in rabbits.
Sato, J; Sasaki, S; Yamada, N; Tsuchitani, M
Veterinary Pathology null
S U Kim et al.
Brain research, 300(1), 195-199 (1984-05-21)
Previous biochemical studies have shown that high enzyme activity of 2':3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) is found in isolated myelin and oligodendrocytes. We report here the specific and intense immunofluorescence staining of cultured oligodendrocytes obtained from the brains of mouse, rat, calf
H J Sheedlo et al.
Brain research, 288(1-2), 330-333 (1983-12-12)
The distribution of 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNP) in fixed-tissue sections and Percoll-isolated cells (oligodendrocytes) of bovine cerebrum was studied by immunofluorescent techniques. Fluorescence in tissue sections exposed to CNP antisera was restricted to oligodendrocyte-like cells. Also, Percoll-isolated cells (oligodendrocytes) were
The Use of Interleukin 12-secreting Neural Stem Cells for the Treatment of Intracranial Glioma
Ehtesham, M., et al.
Cancer Research, 62, 5657-5663 (2002)
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