CC076

Human Collagen Type IV

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Properties

• biological source: human
• Quality Level: 100
• Assay: 95% (Human collagen type IV, SDS-PAGE)
• form: liquid
• mol wt: 300 kDa
• manufacturer/tradename: Chemicon^®
• concentration: 1 mg/mL
• technique(s): cell culture | mammalian: suitable
• impurities: <5% Human collagen types I-III, V, VI, and non-collagen proteins
• input: sample type epithelial cells; sample type hematopoietic stem cell(s); sample type pancreatic stem cell(s); sample type neural stem cell(s); sample type mesenchymal stem cell(s); sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• solubility: water: soluble at 20 °C
• NCBI accession no.: NM_001845.4
• UniProt accession no.: P02462
• Binding Specificity: Peptide Source: Laminin; ; Peptide Source: Nidogen
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... COL4A1(1282)

Description

General description

Collagen type IV is the major structural element of basal membranes. Human collagen type IV is a native triple helix. Collagen was extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type IV was purified by using differential salt precipitation. Purity and retention of native helical structure was controlled by SDS-PAGE and by reaction with anti-collagen type specific monoclonal antibodies. Molecular weight: 300kDa; Solubility: 10mg/mL

Product Source: Human placenta, negative for HBsAg and HIV antibodies

Application

Coating material for cell culture studies

Formation of collagen gels

Optimal working dilutions must be determined by end user.

Physical form

Liquid purified protein. In 100 μL of 0.5M acetic acid solution, pH 2.5. Salt-free, no preservative.

Storage and Stability

Store at -20ºC for up to 12 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Retention of native structure was confirmed by ability to form microfibrils.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable