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Key Documents

ABN899

Sigma-Aldrich

Anti-Olig-2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Oligodendrocyte transcription factor 2, bHLHe19, Olg-2, Oligo2, RACK17, RK17

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_058663

UniProt accession no.

Q9EQW6

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... OLIG2(10215)

General description

Oligodendrocyte transcription factor 2 (UniProt Q9EQW6; also known as bHLHe19, Olg-2, Oligo2, RACK17, RK17) is encoded by the Olig2 (also known as Bhlhb1) gene (Gene ID 50913) in murine species. Olig2 is a basic helix-loop-helix (bHLH) transcription factor expressed in a spinal cord ventricular zone domain where it mediates the sequential generation of motoneurons and oligodendrocytes during embryogenesis. Olig2 also functions as an anti-neurogenic factor in pMN progenitors at early time points to sustain the cycling progenitor pool. The anti-neurogenicity of Olig2 is also responsible for its role in tumorigenesis, especially in brain tumors and leukemia. Due to its expression in glioblastoma and other diffuse gliomas, Olig2 is a useful diagnostic marker of these brain tumors.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence in the N-terminal region of mouse Olig-2.

Application

Anti-Olig-2 Antibody is an antibody against Olig-2 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry (Paraffin).
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Olig-2 in 500 µg of mouse brain tissue lysate.
Immunohistochemistry Analysis: An 1:500 dilution from a representative lot detected Olig-2 in rat hindbrain tissue sections.
Immunocytochemistry Analysis: An 1:500 dilution from a representative lot detected Olig-2 in PC12 cells.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Olig-2 in 10 µg of mouse brain tissue lysate.

Target description

~37 kDa observed. 32.40 kDa calculated.

Linkage

Replaces: AB15328

Physical form

Purified rabbit polyclonal antibody in PBS and 0.1 % sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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