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Key Documents

ABC213

Sigma-Aldrich

Anti-phospho ULK1 Antibody (Ser777)

from rabbit, purified by affinity chromatography

Synonym(s):

Serine/threonine-protein kinase ULK1, Serine/threonine-protein kinase Unc51.1, Unc-51-like kinase 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

inhibition assay: suitable (peptide)
western blot: suitable

NCBI accession no.

NP_033495

UniProt accession no.

O70405

shipped in

wet ice

target post-translational modification

phosphorylation (pSer777)

General description

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

Immunogen

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human ULK1 phosphorylated at Ser777.

Application

Anti-phospho ULK1 Antibody (Ser777) is a highly specific rabbit polyclonal antibody, that targets Autophagy-related protein & has been tested in western blotting & Peptide Inhibition Assay.
Evaluated by Western Blotting/Peptide Inhibition Analysis in A431 cell lysate.

Western Blotting/Peptide Inhibition Analysis: 2 µg/mL of this antibody detected phospho ULK1 (Ser777) in 10 µg of A431 cell lysate.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho ULK1 (Ser777) in 10 µg of A431 cell lysate.

Target description

~120 kDa observed

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hyowon Choi et al.
Autophagy, 12(9), 1631-1646 (2016-06-18)
Nucleus pulposus (NP) cells reside in the avascular and hypoxic microenvironment of intervertebral discs. Importantly, many activities related to survival and function of NP cells are controlled by the HIF-family of transcription factors. We hypothesize that NP cells adapt to
Prasun Guha et al.
Cell reports, 26(10), 2692-2703 (2019-03-07)
Autophagy plays a broad role in health and disease. Here, we show that inositol polyphosphate multikinase (IPMK) is a prominent physiological determinant of autophagy and is critical for liver inflammation and regeneration. Deletion of IPMK diminishes autophagy in cell lines
Ayaka Sugiura et al.
Immunity, 55(1), 65-81 (2021-11-13)
Antigenic stimulation promotes T cell metabolic reprogramming to meet increased biosynthetic, bioenergetic, and signaling demands. We show that the one-carbon (1C) metabolism enzyme methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) regulates de novo purine synthesis and signaling in activated T cells to promote proliferation and
Chao Liu et al.
Scientific reports, 7(1), 4543-4543 (2017-07-05)
Nucleus pulposus (NP) cells reside in a physiologically hyperosmotic environment within the intervertebral disc. TonEBP/NFAT5 is an osmo-sensitive transcription factor that controls expression of genes critical for cell survival under hyperosmotic conditions. A recent report on NP and studies of

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