AB1589P
Anti-Adenosine A2b Receptor Antibody
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ADORA2B(136)
Specificity
Adenosine A2b receptor. Recognizes a band of approximately 50-52 kDa in several rat, mouse and human tissues (thymus, placenta, colon and small intestine). The antibody also recognizes the 35 kDa band in small intestine. No bands were detected in skeletal muscle or heart.
Immunogen
Amino acid sequence corresponding to the 2nd extracellular domain (16 amino acids) of the human brain A2bR gene (Pierce et al. 1992; Tucker & Linden 1993).
Application
Detect Adenosine A2b Receptor using this Anti-Adenosine A2b Receptor Antibody validated for use in ELISA, IC, IH & WB.
Immunohistochemistry: 10-50 μg/mL on frozen and formalin fixed tissue sections. The receptor was localized in epithelial cells of the crypts, and in synctiotrophoplast cells of human placenta and in T84 human colon adenocarcinoma cells.
Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG292)/well)
Optimal working dilutions must be determined by end user.
Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG292)/well)
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
Physical form
Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Characterization of adenosine receptors in bovine corneal endothelium.
Kah Y Tan-Allen, Xing Cai Sun, Joseph A Bonanno
Experimental Eye Research null
Adenosine stimulates fibroblast growth factor-7 gene expression via adenosine A2b receptor signaling in dermal papilla cells.
Iino, M; Ehama, R; Nakazawa, Y; Iwabuchi, T; Ogo, M; Tajima, M; Arase, S
The Journal of Investigative Dermatology null
Diabetes-induced alterations of adenosine receptors expression level in rat liver.
Marzena Grden,Marzena Podgorska,Andrzej Szutowicz,Tadeusz Pawelczyk
Experimental and Molecular Pathology null
A2BR adenosine receptor modulates sweet taste in circumvallate taste buds.
Kataoka, S; Baquero, A; Yang, D; Shultz, N; Vandenbeuch, A; Ravid, K; Kinnamon, SC; Finger, TE
Testing null
A short cerebral ischemic preconditioning up-regulates adenosine receptors in the hippocampal CA1 region of rats.
Ai-Min Zhou, Wen-Bin Li, Qing-Jun Li, Hui-Qing Liu, Rong-Fang Feng, Hong-Gang Zhao
Neuroscience Research null
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