524572

Phosphatase, Alkaline, Calf Intestine

• Synonym(s): Phosphatase, Alkaline, Calf Intestine, Alkaline Phosphatase, CIAP
• CAS Number: 9001-78-9
• Enzyme Commission number: 3.1.3.1 (BRENDA, IUBMB)
• MDL number: MFCD00131849
• UNSPSC Code: 12352202

Properties

• biological source: bovine (calf) intestine
• Quality Level: 100
• form: liquid
• specific activity: ≥1500 U/mg
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• pI: 5.7
• solubility: dilute buffer: soluble; water: soluble
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Native, alkaline phosphatase from calf intestine. Homodimeric enzyme that hydrolyzes phosphate esters of primary and secondary alcohols, amines, and phenols. Useful in hydrolysis of 5′-terminal phosphates of DNA and RNA. Note: 1KU = 1,000 units.

Note: 1 KU = 1000 units.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of pNPP per min at 25°C, pH 9.6.

Physical form

In 6 mM MgCl, 6 mM Tris-HCl, 0.12mM ZnCl, 40% glycerol, pH 7.6.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2